The work presented in this report was performed under the HydroPASSAGE project (www.hydropassage.org) and includes validation and model confirmation for the computational fluid dynamics (CFD)-Biological Performance Assessment (BioPA) modeling tools. The BioPA toolset developed by Pacific Northwest National Laboratory (PNNL) is used in combination with CFD simulations to predict the relative risk of injury and mortality that fish may experience during hydropower turbine passage. Biological response models, often called dose-response models, were developed in laboratory tests using fish exposed to stressors like those associated with passage through hydropower facilities. The BioPA toolset combines the biological response models with particle trajectories and impacts through CFD analysis to predict a relative risk. The resulting relative risk index scores may be used to refine an original design, compare competing turbine designs, or improve the performance of an existing hydropower turbine.