The Biological Performance Assessment (BioPA) toolset uses CFD simulations of the turbine design and operations to quantify the exposure of passing fish to rapid decompression, blade strike, shear forces and turbulence. Simulations are combined with fish dose-response relationships to objectively compare turbine designs and operations. BioPA is a Microsoft Excel-based program that integrates the exposure probabilities of stream traces and combines them with laboratory measurements of fish impacts.